FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465980
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31900.684578737284862No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGAC2450.040.970476
GTTCCGA2100.031.5853961
TAGAGCA1550.031.263347
GTATAGC901.794815E-731.0312651
TCCTACA953.0920273E-729.163492
AGACGTC1800.028.7512119
TCGGTAG1352.928573E-1028.2183763
CATCGGT500.00729690427.9281371
ACTCGTC1503.45608E-1127.6011623
ATCGGTA1404.456524E-1027.2105772
TCCGTTC2200.026.7608153
CCGTTCT2250.026.160494
CTAAGGT809.319349E-525.9681344
TGCGAGA1607.4578566E-1125.9653345
CTTCCGT1801.0913936E-1125.2129021
TCATCCG2500.024.9267235
TCGTCTC1801.2732926E-1124.91771525
CTTCCCA2500.024.84104320
CCTACAT1001.5156831E-524.2421513
GGTAGAG2150.024.1538015
Produced by FastQC (version 0.11.2)