FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences587353
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14500.24687028073407305No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGAC3250.040.471846
GTTCCGA3050.034.3403851
CTCCTAG1206.730261E-1132.0033341
CTTCCCA2700.031.92444420
TCCGTTC3000.030.0168343
CGAGACG3650.029.398257
GTCTCGC2500.029.0932432
CCGTTCT2900.028.6583864
AGTCGTG1503.274181E-1127.6912565
CTTCCGT2900.027.6894041
TTCCCAT3650.027.39379721
TTCCGTT3300.027.2880332
CCCATCT3050.027.13054323
CATCCGA4350.027.0546766
GTACTAG658.071403E-426.8559441
GGTACCG658.4868073E-426.626215
GACCACA2600.026.52184723
TCATCCG4450.025.6688635
CGTTCTG2850.025.5051045
AGACCAC2850.025.40513422
Produced by FastQC (version 0.11.2)