FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss94.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss94.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1723162
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36990.21466350813214313No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC7500.045.71723
CCGTTCT7800.042.1765824
CTTCCCA11700.039.4948920
GCGAGAC13800.038.3775636
TTCCGTT9050.036.7423062
GTTCCGA12300.035.489381
TCTTCCC12300.035.04506319
CTTCCGT7750.034.6962471
ACTCGTC8750.034.68139623
TTCCCAT13350.034.35518621
TCGTCTC7750.034.2738125
GTCTCGC8500.034.229782
TCCCATC13700.033.22579222
GCACTCG8900.032.93448621
GACCACA9700.031.99578323
CCCATCT13550.031.55762923
CTCGTCT9300.031.52893824
GTCTGCT12100.031.34940323
AGACGTC12550.030.77487219
GTTCCGT5300.029.6503721
Produced by FastQC (version 0.11.2)