FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1563568
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT21140.13520358564513985No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20300.12983125773871043No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC16410.1049522630291743No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA16020.10245796792976065No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC6150.039.9889033
TTCCGTT6500.038.371022
CCGTTCT6300.037.9261864
CTTCCGT5600.036.7943951
GCGAGAC8350.034.8199426
GTTCCGA8750.031.9300521
CTTCCCA8050.029.55595620
AGACGTC7350.029.55595619
GACGTCT7600.028.13000920
TCTTCCC7800.027.40872819
TTCCCAT8700.027.34775221
TCCCATC8850.027.27385722
GCACTCG5900.026.8842321
TCATCCG12250.026.001515
AGACCAC6800.025.8614622
CGAGACG10000.025.613387
TCGGTAG3000.025.4014023
CATCCGA12350.025.2238146
TCCTACA3300.025.1930922
GTTCCGT4400.024.6051881
Produced by FastQC (version 0.11.2)