FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7500680
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG141540.1887028909378883No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC31250.041.8833433
GCGAGAC56000.038.8105746
CCGTTCT33600.037.196874
CTTCCGT28950.035.5732351
TTCCGTT37100.034.4418872
GTTCCGA53650.034.356911
AGACGTC45700.030.86546119
TTCCCAT49150.030.6636321
CTTCCCA50650.030.43643220
GACGTCT46250.030.20013820
GTTCCGT18450.029.5174051
TCCCATC50500.029.29776422
CGTTCTG23950.029.1942315
TCTTCCC49050.028.40586319
GTCTGCT46350.027.82835423
GTCTCGC36700.027.646242
ACTCGTC27900.027.44191623
GACCACA38200.027.26523623
AGACCAC41000.026.49691222
TCGTCTC27850.026.38107725
Produced by FastQC (version 0.11.2)