FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1188676
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30970.2605419811622343No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT12920.10869236023945969No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG12450.10473838119050102No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGTT5400.045.534132
TCCGTTC5600.043.9041943
CTTCCGT5350.039.8198781
GCGAGAC8450.039.729416
GTCTCGC4700.039.0526472
CCGTTCT6450.037.5751384
CGTTCTG4300.037.035225
TCTTCCC7350.035.65725319
CTTCCCA7800.035.3670420
TCCCATC7900.035.35584622
GTTCCGA6800.034.9239921
TTCCCAT8000.034.48286421
GTTCCGT3200.032.741241
ACTCGTC5850.031.83167623
CCATCTG5750.031.79089524
GACCACA7150.030.3847823
CCCATCT9000.030.26956623
TCGTCTC5250.030.21864925
GCACTCG6250.029.79319421
CGAGACG10400.029.285087
Produced by FastQC (version 0.11.2)