FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1345025
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG274822.043233397148752No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16360.12163342688797606No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC8000.058.448663
CCGTTCT8350.057.236724
TTCCGTT8700.054.146042
GTTCCGT4000.051.5217061
CTTCCGT7600.046.4200941
CGTTCTG5750.046.370325
CACTCGT10700.045.76403822
GCACTCG11200.044.95257621
GTCGTGT4100.043.9108476
GTTCCGA15700.043.3843571
ACTCGTC10900.043.34248423
TCGTCTC9800.043.29242325
TTGCGTT1800.042.3347662
TTCCCAT18850.042.0761721
CTTCCCA20150.041.58636520
GCGTACT758.403731E-1041.558834
TGCGTTC1850.041.1890453
AGTCGTG4550.041.0959975
CGTTCTA2200.040.923045
TCCCATC19500.040.4967922
Produced by FastQC (version 0.11.2)