FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss89.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss89.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3396919
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1066343.139138731303278No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG188240.554149215804086No Hit
GGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44320.130471171081795No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGC147250.023.76914819
CGGGGGC51100.022.9034881
GGGGGCG183150.020.7480620
GGGGCGG190050.019.86806121
GGGCGGG201550.019.02556822
GAGGTGT24650.018.46327269
GCGGCGG57800.017.6798673
CCGGGGG20600.017.4793787
AGAGGTG31900.017.2934269
CGGCGGG56550.017.271524
GGGGGCC16800.016.9083882
GCGGGGG233700.016.70580524
GGCGGGG234850.016.66560223
CGGGGGG246300.015.75316225
GGCGGCG64600.015.6584222
GGGGGGT53000.015.35484356
GCCGGGG32000.015.1471696
GAGAGGT39800.015.07362969
GGCCGGG33100.014.3327495
TAGGACG3901.200533E-1014.2091954
Produced by FastQC (version 0.11.2)