FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1991614
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40740.20455771048004281No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA21430.10760117171299258No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG20840.10463875027992371No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC9850.050.999093
TTCCGTT10800.046.1957742
CCGTTCT10450.045.741014
GTTCCGT5000.041.2216071
CGTTCTG6400.040.583315
CTTCCGT9200.040.2495421
GCGAGAC19650.039.6449136
CTTCCCA14950.036.67803220
TTCCCAT15350.035.4975821
TCCCATC15550.035.4854722
GCACTCG11050.035.26681521
GTCTCGC10600.034.973692
CTTGCGT3150.034.3790631
GTTCCGA15100.034.2395171
TCTTCCC15700.033.16861719
CGTTCTC5000.032.5532265
GACCACA12050.032.05391323
CCATCTG10000.031.38909724
AGACCAC13500.031.1664322
CTAAGTT5350.031.0763764
Produced by FastQC (version 0.11.2)