FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1324533
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21250.16043390387404466No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC6100.056.760313
TTCCGTT6800.051.4284632
CCGTTCT6900.050.675434
CTTCCGT6600.048.1315731
TGCGTTC2250.038.470883
TTCCCAT11400.038.11363621
AGACGTC9300.037.0793219
CCCATCT10700.036.4188123
GCACTCG6000.036.20795421
TCCCATC12700.035.0281422
GCGAGAC13950.034.734716
GACCACA8500.034.4850723
AGTCGTG3700.033.6803445
GACGTCT10450.033.65879420
TTGCGTT2600.033.293372
CCATCTG6750.032.70313624
CACTCGT6200.032.26022722
GTTCCGT3800.032.1526681
CTTCCCA13750.031.5996720
CGTTCTC4100.031.2387475
Produced by FastQC (version 0.11.2)