FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1590626
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26790.16842425560754068No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24450.15371306642793467No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC8500.055.853043
TTCCGTT8400.055.6946372
GTTCCGT4150.052.1988951
CCGTTCT9650.046.3168374
CTTCCGT8550.042.908261
TTCCGTA2650.041.8468742
TTCCCAT16300.041.68519221
CTTCCCA17950.039.5827520
TCCCATC16900.039.38889322
GCGAGAC22450.038.7193376
GCACTCG10450.038.2864321
GTTCCGA18700.037.742221
TCCGTAC3250.036.2527283
TCTTCCC17950.035.7397619
ACTCGTC11050.035.5832623
GACCACA13400.035.52036723
CCCATCT17900.035.0688523
TCGTCTC10100.034.83557525
GTCTCGC11050.034.811252
AGACCAC14650.034.6085122
Produced by FastQC (version 0.11.2)