FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1606024
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29870.1859872579737289No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26840.1671207902248036No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG16250.10118155145875778No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC9000.051.9491923
TTCCGTT9400.049.7417032
CCGTTCT9700.047.120314
GTTCCGT3250.046.218241
CTTCCGT8250.044.8828281
GCGAGAC15000.040.616746
GTTCCGA14400.040.269281
CGTTCTG6100.039.727635
TCGGTAG6000.037.518863
TCCGTAC1800.036.556843
TTCCCAT13250.035.91860221
TCGTCTC6100.034.48921625
TCCCATC14000.034.48706422
ATCGGTA6000.034.0577132
ACTCGTC6950.033.7427423
AGACGTC11950.033.47698619
GTCTGCT10600.031.8842723
TGCGTTC2500.031.8621713
GTAGAGC8300.031.6970736
GCACTCG7600.030.85684621
Produced by FastQC (version 0.11.2)