FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1262851
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG149041.1801867362024498No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26220.2076254443319125No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13450.10650504295439446No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC7800.055.944563
CCGTTCT7900.053.474354
TTCCGTT8550.053.0666542
CGTTCTG5200.042.6159445
GTTCCGA13350.040.5619661
GCGAGAC12850.038.2463956
ACTCGTC5550.035.4132323
GACCACA7650.034.70667323
AGTCGTG2800.034.6254545
CGTTCTC3300.034.625455
CCCATCT11250.033.71505423
CGTTCTA1850.033.6896325
GTTCCGT4900.033.509681
TTCCGTA2800.033.398142
CTTCCGT11550.033.2720261
GTCTGCT9800.032.72205723
CTTCCCA11700.032.7130320
TTCCCAT11400.032.666521
TCCCATC11850.031.71698222
GTCTCGC8000.031.6045342
Produced by FastQC (version 0.11.2)