FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108027
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5650.5230173937996982No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1350.12496875781054737No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTTA150.002355267368.9592215
CGAAATT208.504193E-568.9592213
CTTGCGT252.4038611E-455.892581
TCCGTTC452.8891009E-853.909743
CCCCTGA200.0069876752.3992961
TTGCGTT407.917788E-752.008622
ATAGCGG200.007210965351.9843983
CCTTGTT200.007358251651.71941814
GCATCAA200.007358251651.71941810
GAGTCTC200.007371751651.6954648
TCTTCCC1100.050.1521619
TCCCATC1250.046.8922722
CCGTTCT605.313268E-946.208354
ATCTATA306.289879E-445.99412526
GGTAAGT306.289879E-445.99412528
CCGACCC306.304275E-445.97281336
CTTCCCA1300.045.08872220
CACTCGT704.3837645E-1044.3309322
ACTCGTC704.3837645E-1044.3309323
GACGTCT803.45608E-1143.09951420
Produced by FastQC (version 0.11.2)