FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1595443
Sequences flagged as poor quality0
Sequence length75
%GC67

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23285914.595256615247301No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG398482.497613515493816No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG316891.9862195014174748No Hit
GGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG181121.1352332863035532No Hit
GGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG94710.5936282273951498No Hit
CGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55270.34642415930873116No Hit
GGGCGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39620.2483322813789023No Hit
GGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32080.20107268012708696No Hit
GGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21510.13482148845179676No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGGC11350.043.3292587
CGTCTAC3100.040.5666471
GGCCGGG31650.040.488385
CTAGTCG1250.038.7986223
GCCGGGG33700.038.2285776
CGGGGCG25750.038.031628
CTACACG3300.037.7872924
TACACGT3450.037.1436925
GGCGGCC18550.036.793862
CGGCCGG17650.036.3064884
GGGGGCC18500.036.1442032
GTCTACA3600.034.6459962
CGGCGGC46450.034.368361
AGTCGTG2750.034.0042765
CCGGGGG24950.032.481357
GGGGCCG21050.032.2553863
TCGGGCG5800.032.252553
GTCGTGT2800.032.15816
GGCGGCG75750.031.9703642
GCGGCCG22150.031.748313