FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022201
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG226792.2186438870633074No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30740.30072363458850065No Hit
GGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17680.1729601125414669No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC4250.051.3569873
TTCCGTT4900.043.841592
CCGTTCT5150.042.3757254
CTTCCGT4350.041.7667581
AGTCGTG3200.040.04325
TTCCCAT9800.038.70322421
GTCGTGT3400.037.674756
TCCCATC9900.037.61569622
CTTCCCA10200.037.18544820
TCTTCCC10150.036.68919819
ACTCGTC4800.036.6361223
GTTCCGA9950.036.1685261
GCGAGAC11550.035.9688726
GCACTCG5000.035.17067321
CCCATCT10100.034.4810523
GTTCCGT2350.034.1961061
GTCTGCT8250.033.85412223
GTCTCGC5950.033.1931572
TCGGTAG3950.032.4528273
TCGTCTC5650.031.7472125
Produced by FastQC (version 0.11.2)