FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1544328
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37070.24003968068959444No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC6100.037.4672163
CCGTTCT6400.034.6265344
CTTCCGT5950.034.022661
GCGAGAC10450.033.7827536
TTCCGTT6850.032.3581162
CTTCCCA9600.029.81526220
GTTCCGA10900.029.4590361
TCCCATC9450.028.82883322
TTCCCAT9750.028.6491821
AGACGTC7500.028.0469919
GACGTCT7950.027.32783320
CGAGACG12900.026.2925937
CCCATCT9850.026.25770823
GTCTCGC6650.026.0400712
TCTTCCC10050.025.39120919
CTATTAG1801.0913936E-1125.2074011
TCATCCG11950.023.7519655
GTTCCGT3400.023.6105541
CATCCGA12200.023.5466696
AGACCAC7900.023.1355922
Produced by FastQC (version 0.11.2)