FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1337140
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21140.1580986284158727No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC6500.040.4884263
TTCCGTT6750.037.9685442
CCGTTCT7050.036.3432964
GCGAGAC10100.034.956666
CTTCCGT5250.034.575311
GTCTCGC6350.034.3607642
GTTCCGA8250.033.8499531
TTCCCAT7350.031.90629621
GACCACA7750.031.59449823
AGACGTC8100.031.50659819
TCCCATC8000.030.17608322
CTTCCCA7550.030.14753720
GTCTGCT8200.029.01950823
TCGTCTC6600.028.74235725
AGACCAC9000.028.73912822
GACGTCT8800.028.60849620
CGTTCTC3400.028.510965
CCACACG8600.028.47498525
CGTTCTG5250.028.35585
CCCATCT8900.028.28705223
Produced by FastQC (version 0.11.2)