FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2356132
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41360.17554194756490724No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36980.1569521571796487No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTCT16750.053.744454
CTTCCGT13750.052.577711
TCCGTTC17000.052.354253
TTCCGTT17150.052.3046722
GTTCCGT6050.051.376991
CGTTCTC6300.042.8648575
CGTTCTG11200.041.1131675
CACTCGT15950.041.07819722
GCGAGAC35750.040.574046
GACCACA21200.040.50509323
TTCCCAT29250.040.32154521
GCACTCG17050.040.04771421
TCCCATC29800.040.03854822
TCGTCTC14650.039.31950825
AGTCGTG8800.038.9493185
TCCGTAC3300.038.8289383
GTCGTGT8700.038.1998986
CTTCCCA31650.038.026720
TTCCGTA3300.037.7827342
TGCGTTC4500.036.9399643
Produced by FastQC (version 0.11.2)