FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1251315
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24650.19699276361267948No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT12750.10189280876517902No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC5700.041.3089873
TTCCGTT5650.039.226262
GTTCCGA9300.039.0357781
CCGTTCT5800.038.8026124
TCCCATC10400.035.47591822
GCGAGAC11200.035.2265976
CTTCCCA10450.034.97621520
TTCCCAT10700.034.1590121
CCCATCT10150.033.63197323
CTTCCGT6400.032.725311
TCTTCCC10500.032.51090619
AGACGTC7550.031.5126819
CGTTCTG4250.030.9516015
ACTCGTC6050.030.20672823
TCGTCTC6000.029.320825
TCATCCG11650.029.1198145
GACGTCT8050.028.69869420
GCACTCG6400.028.554821
GTCTGCT7500.028.50451323
CTCGTCT6300.028.47211524
Produced by FastQC (version 0.11.2)