FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences928525
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29830.3212622169570017No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT12410.13365283648797824No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA11660.125575509544708No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA10800.11631350798309147No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG9830.10586683180312863No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC9700.10446676179962844No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC9540.10274359871839744No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCGA5800.041.5271641
TCCGTTC2750.039.0407183
AGTAGAG5200.036.623025
GCGAGAC6850.036.390646
TTCCGTT3100.035.7578282
TTCCCAT4700.033.75350621
GTCTCGC3400.033.621562
TCCCATC5100.031.78239422
ACTCGTC3400.031.44428323
CCGTTCT3600.030.783134
TAGAGAA5300.030.6988967
GTAGAGA6100.030.6486746
CTTCCGT3550.030.4820751
CGTTCTG2050.030.402795
CTTCCCA5650.029.29892720
AGAGTAG4750.029.1645433
CTCCTAG1750.027.9255141
GAGTAGA5000.027.7048174
AAGCTTG2250.027.6973196
TCATCCG7200.027.4117745