Basic Statistics
Measure | Value |
---|---|
Filename | HW32WAFXX_n02_zt2ss75.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 928525 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 2983 | 0.3212622169570017 | No Hit |
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT | 1241 | 0.13365283648797824 | No Hit |
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA | 1166 | 0.125575509544708 | No Hit |
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA | 1080 | 0.11631350798309147 | No Hit |
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG | 983 | 0.10586683180312863 | No Hit |
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC | 970 | 0.10446676179962844 | No Hit |
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC | 954 | 0.10274359871839744 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTTCCGA | 580 | 0.0 | 41.527164 | 1 |
TCCGTTC | 275 | 0.0 | 39.040718 | 3 |
AGTAGAG | 520 | 0.0 | 36.62302 | 5 |
GCGAGAC | 685 | 0.0 | 36.39064 | 6 |
TTCCGTT | 310 | 0.0 | 35.757828 | 2 |
TTCCCAT | 470 | 0.0 | 33.753506 | 21 |
GTCTCGC | 340 | 0.0 | 33.62156 | 2 |
TCCCATC | 510 | 0.0 | 31.782394 | 22 |
ACTCGTC | 340 | 0.0 | 31.444283 | 23 |
CCGTTCT | 360 | 0.0 | 30.78313 | 4 |
TAGAGAA | 530 | 0.0 | 30.698896 | 7 |
GTAGAGA | 610 | 0.0 | 30.648674 | 6 |
CTTCCGT | 355 | 0.0 | 30.482075 | 1 |
CGTTCTG | 205 | 0.0 | 30.40279 | 5 |
CTTCCCA | 565 | 0.0 | 29.298927 | 20 |
AGAGTAG | 475 | 0.0 | 29.164543 | 3 |
CTCCTAG | 175 | 0.0 | 27.925514 | 1 |
GAGTAGA | 500 | 0.0 | 27.704817 | 4 |
AAGCTTG | 225 | 0.0 | 27.697319 | 6 |
TCATCCG | 720 | 0.0 | 27.411774 | 5 |