FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6694
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG590.8813863161039737No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG190.2838362712877204No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGAGA200.0070674651.70488720
AAGACCA200.0070674651.70488721
AGACCAC200.0070674651.70488722
TGTGCTC901.8448959E-422.97994814
CGTGTGC1003.7657339E-420.68195512
GTGTGCT1003.7657339E-420.68195513
GAGACGT1003.7657339E-420.6819558
GTGCTCT1003.7657339E-420.68195515
GACGTGT1055.2355626E-419.697110
ACGTGTG1055.2355626E-419.697111
AGACGTG1107.164336E-418.8017779
CTCTTCC950.00710062918.14206718
TGCTCTT1159.6627726E-417.9843116
Produced by FastQC (version 0.11.2)