FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1111
Sequences flagged as poor quality0
Sequence length75
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG181.6201620162016201No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60.54005400540054No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40.36003600360036003No Hit
CATCTGACGCTGCCGACGACGGAGAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAGGGGGGGGGGG40.36003600360036003Illumina Single End PCR Primer 1 (100% over 32bp)
GGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30.27002700270027No Hit
CATCTGACGCTGCCGACGACGGAGAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAGAGGGGGGGGG20.18001800180018002Illumina Single End PCR Primer 1 (100% over 32bp)
CATCTGACGCTGCCGACGACGGAGAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAGGGGGGGGGGGG20.18001800180018002Illumina Single End PCR Primer 1 (100% over 32bp)
GGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.18001800180018002No Hit
CATCTGACGCTGCCGACGACGGAGAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGGGG20.18001800180018002Illumina Single End PCR Primer 1 (100% over 32bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTGC150.001487043771.9047557
GACGCTG150.001487043771.9047556
TGACGCT150.001487043771.9047555
GCTGCCG351.0550139E-968.636379
CTGCCGA251.5397545E-668.6363710
CGCTGCC304.1476596E-868.636368
ATCTCGG150.001814612468.6363634
TGCCGAC205.4205615E-568.6363611
CCGTATC205.4205615E-568.6363647
TCTCGGT150.001814612468.6363635
GATCTCG150.001814612468.6363633
AGTAAAA150.001814612468.6363654
CGTATCA150.001814612468.6363648
GACGACG150.001814612468.6363615
CGACGGA150.001814612468.6363617
ACGACGG150.001814612468.6363616
GCCGTAT205.4205615E-568.6363646
AGATCTC150.001814612468.6363632
GCCGACG205.4205615E-568.6363612
CCGACGA150.001814612468.6363613
Produced by FastQC (version 0.11.2)