FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1674801
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61770.36881993741345986No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT23310.13918071460430223No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA19970.11923804678884238No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC18970.11326718816145918No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC18250.10896816994974327No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG17630.10526623760076571No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC7550.039.911183
CTTCCGT6950.039.6771471
TTCCGTT8050.037.4322242
GTTCCGA10550.032.7552451
GCGAGAC6800.031.5548616
CCGTTCT9900.031.4802634
GTTCCGT3450.029.3411431
AGACGTC5950.028.97767319
GTCTCGC3850.027.8883742
TCGGTAG4250.026.8934963
CATCCGA13000.026.8881876
CTCGTCT4250.026.78337124
ATCGGTA3900.026.642742
ACTCGTC4150.026.58963423
CGAGACG7450.026.479037
TCATCCG13300.026.2887825
TTCCGTA3300.026.2390632
GACGTCT6500.025.99520120
ATCCGAT11700.025.4387687
TCCGTAC3300.025.18953
Produced by FastQC (version 0.11.2)