FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220815
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3390.15352217919978262No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT2920.13223739329302808No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC2700.12227430201752598No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC2590.11729275637977492No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA2590.11729275637977492No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC2320.10506532617802233No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAAGA200.006977417552.4372561
TAGCCAT200.00722809751.9714134
TAGCACT200.00722809751.9714134
TACAGTG200.007234611551.9595765
GTACCCT200.007365821451.72398811
TATTGAG350.001318972239.588255
CTTCCCA557.38125E-637.62597320
TAGATAG655.4267E-737.15201624
TCCTACA400.002535245134.6555062
TGAACCG400.002540918334.6397175
ACCTGGG400.002601096434.47484233
CGTTCTG553.1724316E-431.4906525
GTTCCGA1001.2651071E-831.4623531
GCACCTT450.004631178530.64430455
AATGTTA605.409737E-428.74858325
TTAGAGG854.4319604E-628.39104763
CAGGACC500.00756230427.7180884
AAAAGCG500.007570718427.7117755
GGACCTT500.007570718427.7117756
GATCTAG755.9744325E-527.7117756