FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences552713
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14460.26161859771707924No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9940.17984017021492166No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCGT2000.054.146031
TTCCGTT3650.054.110882
TCCGTTC4000.051.0993733
TTGCGTT750.050.8199272
CCGTTCT4150.048.4000054
CACTCGT3900.044.21776222
GTCGTGT2200.044.055836
CGTTCTG2650.043.113685
CTTCCGT3000.043.0839421
GCACTCG4350.042.0183121
GCGAGAC8950.041.770416
AGTCGTG2350.041.2512555
GTTCCGA6900.040.501941
TTCCCAT6450.040.1008521
TCCGTAC1050.039.5927353
GACCACA5900.039.16644323
TCCCATC6800.039.05468422
ACTCGTC4200.038.59578723
TCGTCTC3600.038.3324825
GTCTGCT6500.037.6735323
Produced by FastQC (version 0.11.2)