FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss69.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss69.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1651781
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT17270.10455381191574427No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17210.10419056763578223No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGAC7850.033.9579126
CCGTTCT6000.032.327214
TCCGTTC6300.030.7925053
GACGTCT6750.028.09983620
GTTCCGA7950.027.2366581
AGACGTC7050.026.90328219
CTTCCCA6850.026.68272420
TTCCGTT7150.026.1636562
TCCCATC7300.025.98272522
TTCCCAT7350.025.80597321
ACTCGTC6650.024.8922723
GTTCTCA6900.024.8014031
TCATCCG9800.024.7341845
ATCCGAT9100.024.7266587
CGAGACG9550.023.9240157
CTTCCGT3950.023.872391
GTTCCGT4550.023.0270941
TCTTCCC7850.022.84364519
CTCGCGT8850.022.21008129
CATCCGA11100.022.143966
Produced by FastQC (version 0.11.2)