FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543774
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40480.2622145469479341No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16040.10390121870170116No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC9050.049.3552863
CCGTTCT9350.047.3936544
TTCCGTT9300.047.285442
CTTCCGT8700.043.338521
GTTCCGT3600.037.8208851
GCGAGAC14950.036.577216
GTTCCGA14350.035.7630841
TCCCATC12150.035.48060222
TTCCCAT11250.035.2535321
CACTCGT7050.033.75337622
CGTTCTG5950.032.5788965
CTTCCCA12100.032.49096720
TCCGTAC2700.032.060393
GACGTCT11100.031.06849320
AGACGTC11700.030.94899719
CCCATCT13400.030.88400823
CGTTCTC5850.030.7689595
ACTCGTC7850.030.75286723
GTCTGCT10100.030.73112123
GTCGTGT4200.030.4892756
Produced by FastQC (version 0.11.2)