FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168470
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11190.6642132130349617No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2190.12999347064759303No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1720.10209532854514158No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGAC1000.051.9044276
ATCACCC200.00726208651.9044235
CATTTCT200.007356697751.7348561
TCTGTCC350.001348450839.4053278
ATGCCTA350.001348450839.40532743
GTTCCGA1450.038.515391
AGGGGTG459.8055825E-538.4477235
ACCGGTG655.439524E-737.13194331
CCTCACC501.8192864E-434.6132663
ATATAGT400.002548747734.6132624
AACACCA501.8224778E-434.602955
TGCGAGA601.3087631E-534.6029475
TATAGTG400.002552477634.6029475
TTACCGG709.692976E-734.48990229
CACTCTA905.164111E-934.47966449
GATTGCA400.002597574634.4796642
CGAGACG1350.033.3213587
AGCGAGA1056.148184E-1032.9551935
GGAGTTC751.6657141E-632.1810237
CGAATTA1101.0513759E-931.34514848
Produced by FastQC (version 0.11.2)