FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191436
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14190.7412398921832885No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA3520.18387346162686224No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC3480.18178399047201155No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT3290.17185900248647068No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC3280.171336634697758No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC3120.16297875007835516No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG2800.1462629808395495No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTGCAACCA2410.1258906370797551No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2340.12223406255876638No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTA2290.11962222361520299No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAACAAC2090.10917486784094946No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTACTAACCACAATTGTAAGTTGTACTAC2020.10551829331996072No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGATGGTAGTATCTAGGACTACCATGGT1950.10186171879897198No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGTCT309.101957E-657.4947668
GTTCCGA1000.055.8971631
CGGTAAC252.5695437E-455.166125
TTCTGCA200.006993725452.4035951
GCTTTAT200.006993725452.4035951
GAGTTAG200.007359954551.7317565
ACCGAAT200.00736757451.7182257
TAACCCC200.00736757451.7182243
TTGAACC506.670962E-848.5114364
GAATTGT306.204044E-446.140726
TGAACCG551.4235411E-744.054985
CTTCCGT602.6219823E-740.758351
GTATAGA350.001264479139.9265481
ACTGAAC350.001314712339.6115883
AACGTGC350.001349071239.40435422
AGTCAAC350.001349071239.40435416
GAATAGT350.001349071239.40435424
TAACTTG350.001349071239.40435422
GACGTCT350.001350807639.3940520
AGTCTAA459.2725306E-538.817471
Produced by FastQC (version 0.11.2)