FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2538771
Sequences flagged as poor quality0
Sequence length75
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1692776.667675028586667No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG286031.1266474999123592No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG222900.8779838748748903No Hit
GGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG125470.4942155082124382No Hit
GGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG68540.2699731484249662No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA36550.044.4290661
TCCGATC41600.042.8801883
CCGATCT40350.041.4550634
TTCCGAT48950.036.5137982
CGGGGCG19850.032.6555868
GTTCCGA9500.032.3489881
CGATCTG29700.031.126785
CGATCTA9150.029.8940935
TTCCGTT7400.029.021512
GCCGGGG28700.028.1039546
CGGGGGC65000.027.8840331
TCTTCCG14500.025.550255
GGCCGGG30900.025.5479325
TCCGTTC8500.025.2647823
GGGGGGC231750.024.42130519
GTCTTAG2450.024.2317311
GGGGGCC19300.024.2290552
GGGGCCG20450.024.051263
GCGGCGG68300.023.023853
GGCGGCG67350.022.8352282
Produced by FastQC (version 0.11.2)