FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1089933
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23070.2116643867100088No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18070.16579000727567658No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC7850.056.0298353
TTCCGTT8650.052.051422
CCGTTCT8600.048.7250374
CTTCCGT7200.046.080471
TTCCCAT13200.042.84857621
GTTCCGA11700.040.5955921
AGTCGTG3650.039.8475535
GTTCCGT4650.039.8060151
TCCCATC13900.039.69827722
GCACTCG6950.039.69827721
CGTTCTG5800.038.8088345
CTTCCCA14850.038.55210520
GTCGTGT3600.038.4700366
CACTCGT7350.038.0070522
CCCATCT13800.037.73499723
GACCACA8800.036.8376323
TCTTCCC14100.035.95544419
GCGAGAC16150.035.3733756
GTCTCGC7500.034.634212
AGACCAC9500.034.48787322
Produced by FastQC (version 0.11.2)