FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences926569
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT9560.10317634196697709No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGAC5200.035.28586
TCCGTTC3650.034.169883
CCGTTCT3700.031.8250874
TCCTACA2050.030.4244822
CTTCCGT2500.029.3450971
GTTCCGT2500.029.3450971
AGACGTC4350.029.32917819
TCGGTAG2350.028.0104183
GACGTCT3950.027.93446220
CATACTA2350.026.5160317
GTTCCGA4250.026.3037241
CGGTAGA2650.026.138254
TTCCGTT4600.025.6109472
ACTCGTC3100.025.58312423
TCCCATC4650.025.21235522
CGAGACG6600.024.12787
CGTTCTG2600.023.9729165
TCTTCCC5200.023.8718819
CTTCCCA5350.023.8470920
TGAACCG1752.2919266E-1023.74465
Produced by FastQC (version 0.11.2)