FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8879444
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG172540.19431396830702463No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG171680.19334543919641817No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTA122040.13744103797490023No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAG112360.1265394544973762No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC39450.049.324993
TTCCGTT39350.048.7505572
GTTCCGT18650.042.3006021
CCGTTCT47600.039.781794
CTTCCGT38950.038.9851041
GCGAGAC66550.038.32516
CGTTCTG27250.034.802025
ACTCGTC32800.034.69690323
CGTTCTC17950.034.1294445
GCACTCG34150.033.83021521
GACCACA42350.033.55014823
GTTCCGA63200.032.5875631
TCGTCTC31700.031.55247925
CTTCCCA63400.031.44030620
AGACCAC47450.031.39772422
TCTTCCC59300.030.88076619
TCGGTAG29050.030.8696233
TTCCCAT64800.030.76121721
TCCGTAC11900.030.5506213
GTCTGCT49400.029.59985423
Produced by FastQC (version 0.11.2)