FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1695346
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33530.19777673702005374No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC9450.046.545413
TTCCGTT9650.045.945092
CCGTTCT9800.045.228434
CTTCCGT7600.044.11881
GCGAGAC17650.042.1676836
GTTCCGT3900.041.19641
GTTCCGA14100.038.6430631
CGTTCTG6850.037.9082685
AGTCGTG4800.037.5081255
TTCCCAT14950.037.36818321
TCCCATC15050.037.1198922
ACTCGTC8850.037.01761623
GTCTCGC8950.036.379882
CTTCCCA15100.035.8550920
GTCTGCT14650.035.30870423
AGACGTC16050.033.9486919
GACGTCT16400.033.8540120
CCCATCT15500.033.59490223
TCGTCTC8850.032.34935425
CGTTCTA2950.031.6887425
Produced by FastQC (version 0.11.2)