FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132460
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28112.122150083043938No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5600.42276913785293674No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3880.29291861694096333No Hit
CGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2640.19930545070209874No Hit
GGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2390.1804318284765212No Hit
GGGGGGCGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1600.12079118224369621No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT1480.11173184357541899No Hit
GGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1440.10871206401932658No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC1330.10040767024007248No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGCT150.002355992868.96258565
TAACCCA252.5228763E-455.358365
AATCTAC200.006972196552.435351
TACCACC200.00736049351.7219421
CGAGGCT200.00736049351.7219454
ACCAGAG200.00736049351.7219411
ATCGCAC200.00736049351.7219459
CAACCGA306.202857E-446.1319666
TCCGTTC551.419885E-744.0517653
AACCCAT404.894418E-543.2487226
ATCCGAT758.3491614E-1041.518777
CCACACC350.001324698939.5416874
CTTTTAG350.001344587739.4220925
GGTCAGA350.001347090239.40719210
TATATAG459.8007804E-538.4433065
TTTCAGC459.999596E-538.312559
CGGGGCG557.3723513E-637.6159558
CCGTTCT655.2826726E-737.2604374
TCATCCG1051.4551915E-1136.2465445
CGGGGGC1500.034.9568941
Produced by FastQC (version 0.11.2)