FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1015246
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG108771.071365954655325No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC4600.054.9753383
TTCCGTT4950.053.8928832
CCGTTCT4950.051.7752044
CTTCCGT4700.051.266331
TCGTCTC3400.039.55805625
GCGAGAC9300.038.71456
AGACGTC8150.037.23329519
GTTCCGA10800.037.183911
GTTCCGT2950.036.6961631
ATCGGTA2550.033.9661032
GACGTCT8550.033.8781420
TTCCCAT8450.033.46290221
GACCACA7000.033.0052623
GTCTGCT7900.032.7371323
TCCCATC8700.032.50132422
GCACTCG4850.031.99463521
GTCTCGC6300.031.895792
ACTCGTC4650.031.8876123
CGTTCTG4150.031.7046725
CACTCGT4700.031.54837822
Produced by FastQC (version 0.11.2)