FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154713
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5740.3710095467090677No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC601.8189894E-1257.7011573
TAGGCTT309.127847E-657.4584823
TCTTTCT200.00736806651.7126321
TTCCGTT751.6370905E-1146.1759262
GCGTTCT306.186949E-446.1609274
AATAGGC306.316874E-445.96678521
ATAGGCT405.0031485E-543.0938622
CGCGAAA350.001348985939.400121
TAATGTG459.7909724E-538.4549455
CGTTCTC655.277834E-737.2717175
AACTAAT655.2912947E-737.2596177
CTTCCGT852.6429916E-936.92111
AGACGTC754.0077794E-836.7734319
CTTGCGT400.002457367534.8699261
GTCTAGA501.8128289E-434.6319472
TACCCAG501.81977E-434.609455
CGTTCTG601.3063802E-534.6094475
ACACCGA501.8232487E-434.5982176
AGCTTGT400.002553541234.5982137
ACCGAAC501.8583398E-434.4862488
Produced by FastQC (version 0.11.2)