FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133984
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1740.12986625268688798No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT1420.1059828039168856No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC1400.10449008836876045No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA1390.10374373059469787No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGAG150.00232690669.179332
TCGGTAA150.002354680668.9727553
ATCGGTA200.00726988351.8845022
ACTAGAC200.007356404351.72956516
AACTAAC451.8209212E-646.102297
TTGTAAC306.303665E-445.98183419
TAACTTG306.303665E-445.98183422
ATAGAAC551.4273428E-744.0232123
CTCGCGT551.4613215E-743.8917529
TCGCGTC551.4613215E-743.8917530
TATAGAA404.9025388E-543.2370872
CTTCCCA404.989909E-543.1079720
TAGATAG651.1294105E-842.4447724
AAGTAGA602.8939212E-740.23410421
GACGGTC602.8939212E-740.2341048
CGGTCCA655.42017E-737.13917510
TAGAACT952.0918378E-1036.4101754
CTCAGGG400.00255542534.589672
GCGAGAC501.8286238E-434.576726
CCGTACA400.002593206734.48637817
Produced by FastQC (version 0.11.2)