FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65381
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA2090.3196647344029611No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA1520.23248344320215353No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG880.13459567764335206No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTGTG150.00230891369.274277
TCATCCG150.00230891369.274275
TCAGCGC150.002344424669.00844654
GCGCCGA150.002358742868.9026827
GTTCTCA850.057.6714321
ACAGGTC252.5573449E-455.16441722
GTCATCA200.00691076652.5221941
CAGTAGT200.00691076652.5221941
CTCATCC200.007213911551.9557044
CCGTTCT200.007213911551.9557044
GTAGAGA1300.050.62356
GAAGAGT900.046.6863981
GCACAGG306.2874844E-445.9703520
GGTCTCC306.2874844E-445.9703525
AGTAGAG1400.044.533465
GAGTAGA1200.043.2964174
AAGAGTA1150.042.1994552
CTCACTT758.1308826E-1041.564564
ACTCTTG602.540546E-740.8505971
AGAGTAG1200.040.4411473
Produced by FastQC (version 0.11.2)