FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4438533
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG146360.3297485903563182No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT48020.10818889935030336No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC18250.037.7546543
GTTCCGA28250.037.3142241
GCGAGAC31550.037.1872256
CCGTTCT18350.036.4122474
TTCCGTT21300.032.6750642
CTTCCGT17650.032.2350851
AGACGTC27000.031.5500919
TCCCATC27450.031.2848622
TTCCCAT26500.030.58365821
CTTCCCA27850.030.3394920
GACGTCT28350.029.56110420
CCCATCT27300.029.43477623
TCTTCCC26550.029.35697719
GTCTCGC20000.029.0856822
CGTTCTG14800.029.0012935
GTCTGCT27650.028.1890723
ACTCGTC17650.027.74668323
TCGGTAG13950.027.302273
GACCACA22600.027.01047723
AGACCAC24250.026.02654822
Produced by FastQC (version 0.11.2)