FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656686
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG69721.0616946303103767No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12660.19278620223363982No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9970.15182294125350623No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT8160.12426030096575837No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC7070.10766180488087151No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCGT1300.048.3119161
TCCGTTC2550.043.44753
TTCCGTT2900.038.206762
GTTCCGA3750.038.148521
GCGAGAC5050.033.5784266
CCGTTCT3200.033.5377164
TCGGTAG1900.032.8000033
CTTCCGT2500.032.1005821
CATGATA1251.2551027E-1030.34909821
ATCGGTA2200.029.9033012
TCCCATC5300.029.28193522
CGTTCTA854.3119E-628.5058775
CTTCCCA4850.028.4433920
GTAGTAA1004.5407432E-727.913551
GTCTGCT3600.027.78168923
TAGTGTA500.00762295427.6808327
CCCATCT5000.027.5900923
TTCCCAT5200.027.19215421
TCGTCTC1800.026.8339325
CATCCGA6800.026.4636676
Produced by FastQC (version 0.11.2)