FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences777327
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90101.1591003528759454No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16080.20686274888174475No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT11180.14382621470758122No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA9860.12684494427699025No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC9690.12465796247911111No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC8570.11024961181073088No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGT2900.042.1294251
TCCGTTC3250.041.5393643
GTTCCGA4800.039.270641
GCACTCG3900.038.90571221
TTCCGTT3350.038.235222
GCGAGAC6550.038.039036
TCCCATC5200.037.13726822
ACTCGTC4150.035.7333423
GTCTCGC3300.035.6674162
TTCCCAT5350.034.80689621
TTCCGTA905.014954E-934.6183742
TCGTCTC4100.034.49349625
CCGTTCT3900.033.7224
AGACGTC5400.031.93019119
GACGTCT5550.031.06521420
AGTCGTG2250.030.7619425
GTCTGCT5050.030.73083923
CTTCCCA5950.030.71540520
GTCCTAC1151.5006663E-930.3541181
CGTTCTG2400.030.281295
Produced by FastQC (version 0.11.2)