FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2821596
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70840.2510635824547526No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32680.11582097507935225No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC16550.051.8800433
CCGTTCT17550.048.324354
TTCCGTT17900.047.583012
GTTCCGT7300.045.4088171
CTTCCGT15050.042.8918381
GCGAGAC33000.042.1565936
CGTTCTG11100.042.096065
TCCCATC29350.039.24732222
CTTCCCA28850.039.09002320
TTCCCAT29400.038.12481721
CCCATCT30800.035.60804423
GCACTCG15500.035.15580721
GTCTGCT21750.034.72614323
AGTCGTG7500.034.6123165
GTTCCGA26650.034.565761
TCGTCTC13900.034.4913525
ACTCGTC15800.034.2700123
GACCACA21000.033.99560523
CCATCTG20600.033.8228224
TCTTCCC30750.033.53436319
Produced by FastQC (version 0.11.2)