FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2387730
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG163600.6851695962273792No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA42050.036.7107961
TTCCGAT45200.036.0252342
TCCGATC48300.033.923253
CCGATCT48500.033.132174
GTTCCGA9000.026.3878441
TCCGTTC8400.025.980333
CTAGCAC4300.024.167753
CTACTAA4550.023.7951161
CTTCCGT9400.023.0356961
TAACGGG18900.022.44404428
TAGGACG3100.022.3406725
GAGGTGT11900.022.3110369
TAACTAG4050.021.3753345
GGTAACG19550.021.16860826
TCAGGGT20200.021.16591538
CCGTTCT9700.021.0646344
CATGGTT18800.020.90674613
AGGGTTC20850.020.50477640
GGGTTCG20550.020.46856541
AATCAGG20950.020.40775736
Produced by FastQC (version 0.11.2)