FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2968941
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG571181.9238509623465068No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT47100.030.2987582
TCCGATC50700.028.0771453
CCGATCT50950.027.8676224
CTTCCGA48050.027.6221851
CGGGGCG12550.025.2770128
CATCCGA8950.024.3665436
GCCGGGG17400.024.2709626
CTTCCGT7800.024.1805931
CGGGGGC31550.023.4695261
GGCCGGG17300.023.0156765
AGAGTGT19150.022.86584769
GTTCCGA10900.022.4305211
GGGGGGC109100.022.37828319
CCGGGGC8800.021.6353447
TCATCCG10100.021.596895
TGAACCG6200.021.2209055
TTCCGTT10350.020.4144652
GGGGGCC10200.020.3750922
TCCGTTC10400.019.9819093
CGGCGGG35750.019.7612464
Produced by FastQC (version 0.11.2)