FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1464153
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG103190.7047760718995898No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA20370.13912480457984922No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG15230.10401918378748669No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCGA7100.036.389451
TCCGTTC4950.034.2733463
TTCCGTT5350.031.7119372
CCGTTCT5900.027.5763594
TCCTACA3100.026.8058762
CGTTCTG4050.026.4934275
TTCCCAT6150.026.35770421
GTTCCGT3100.025.9040831
GCGAGAC8350.025.6949216
CTTCCGT5200.025.5142251
TAGGACG2450.025.4295045
GCACTCG6000.025.29218121
CTACTAT3800.024.8074591
CTTCCCA6450.024.06150420
TCCCATC7050.023.48210122
GTCTCGC5250.023.082842
CTAAGTT4250.022.806584
AGACGTC6100.022.61518519
CTACTAA4800.022.5487561
TCATCCG10550.022.3094025
Produced by FastQC (version 0.11.2)