FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2154987
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT36850.1709987113611358No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA28680.1330866497106479No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC27640.12826063451890893No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC27340.12686851475206115No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26670.12375944727276778No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC24770.11494268874939848No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG23320.10821410987630088No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCGA11000.033.9650231
TCCGTTC7950.033.5392763
CTCCTAG2950.033.1418571
TTCCGTT8400.032.5677722
GTCTCGC7050.028.980322
GCGAGAC11150.028.8672436
GTCCTAC2550.028.7554341
CTTCCGT6850.028.5455421
CCGTTCT9500.028.0605354
CTTCCCA10550.026.47667720
ACTCGTC6700.026.24981723
AAGCTTG5250.025.7100266
CTATAGT3750.025.1404651
GACCACA9550.024.91590323
AGACGTC9150.024.87446419
TCGTCTC6950.024.81630925
TGAACCG4900.024.725795
CATGATA4900.024.63218321
GACGTCT9100.024.63218320
TCCTACA4700.024.3139932
Produced by FastQC (version 0.11.2)