FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1374440
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21700.15788248304764121No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGAATTAAAAGTGAAACCGCAAAAGGCT21080.15337155496056576No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA18160.13212653880853292No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCC16610.12084921859084427No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC15940.11597450598061756No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC14560.10593405314164314No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG14170.1030965338610634No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCGA7800.036.2352681
TCCGTTC4900.036.0411763
TCCTACA2150.032.213082
GCGAGAC8350.031.9158786
AGACGTC5350.030.94345319
GTCCTAC1151.5097612E-930.3419321
TTCCGTT5600.030.300432
CTTCCCA6150.029.72227520
CCGTTCT6100.027.2451134
AAGCTTG3200.027.0391226
GACGTCT5800.026.75874920
CTTCCGT5250.026.5853141
TCTTCCC6750.026.56934519
CGTTCTG4700.026.5127545
TCCCATC7350.026.27737422
CTACTAA4650.025.5133231
ATCGGTA3400.025.4625472
CATGATA3400.025.35959821
CCCATCT7500.025.29197323
TTCCCAT7550.025.12447721
Produced by FastQC (version 0.11.2)