FastQCFastQC Report
Sun 21 Jan 2018
HW32WAFXX_n02_zt2ss44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW32WAFXX_n02_zt2ss44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7836203
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG149800.19116401144789127No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTC32000.048.70043
CCGTTCT32650.047.831414
CTTCCGT28450.044.302771
TTCCGTT38850.039.938122
GCGAGAC65700.038.042756
CGTTCTG22600.036.9202275
GTTCCGA57900.034.6733061
AGACGTC50800.033.80567619
GTTCCGT20100.033.5248761
TTCCCAT56300.033.26011321
TCCCATC56700.032.6609722
GACGTCT52700.031.80205520
CTTCCCA58950.031.41376520
GTCTCGC36050.031.031152
CCCATCT57900.030.85240723
GACCACA44100.029.87188523
ATCGGTA25950.029.628952
GCACTCG36200.029.43623221
CACTCGT35400.029.41992222
TCGGTAG27300.029.303493
Produced by FastQC (version 0.11.2)